Four fire engines and around 25 firefighters tackled a fire at a terraced house on Kittiwake Road in Northolt.
Part of the second floor was damaged by the fire. Two people were rescued by firefighters wearing breathing apparatus from the second floor using an internal staircase. A third person was led to safety from the ground floor. All three people were taken to hospital by London Ambulance Service.
Control Officers took the first of three calls at 0534 and mobilised crews from Northolt and Southall fire stations to the scene. The incident was over for firefighters at 0717.
The cause of the fire has been recorded as undetermined.